I understood that there is no test to detect a patient’s lime disease condition. Hence, with my personal experience, I can state that lime disease is a clinical diagnosis based on indications and symptoms, along with the traveling history to endemic regions. One major thing here is that, no test can forbid lime disease.Similar to the customs, my doctor also diagnosed this disease based upon the Lime disease blood test results, and disease history.
Here, I came to know that, limes disease occurs in three stages: early, early disseminated and late limes disease.
Antibodies are the immune system’s response to “fight back” infection. Tests attempt to be both precise and receptive (detecting only the limes disease antibodies).While analyzing the particular test procedure, I found that fake negative tests emerge due to test sensitivity. A low detectable antibody level alters the bacterium, which restricts the immune system detection or bacterial variations. Further, I also discovered that fake negative tests also emerge because of the test malfunction or cross-reaction of antibodies.
Types of Lyme Diagnostic Tests:
Titer (IFA, EIA, ELISA): As per my knowledge, these tests are mandatory to gauge the level of Bb ( Borrelia burgdorferi) antibodies in fluid. As my tests progressed, I realized that laboratories use different detection techniques, types of measurements, cut-off points, and reagents to diagnose possible limes disease condition.Western Blot: This test generates bands signifying the reactivity of immune system’s Bb. Here too, laboratories differ in their analysis and treatment approach to report these bands.C6 Lime Peptide ELISA: It recognizes antibodies to a reliable surface protein present on almost every identified strain of the lime disease bacteria, (Bb) Borrelia burgdorferi.
I also found that, the test is a bit sensitive to diagnose all stages of lime disease, counting those patients who indicate late stage lime disease.
Direct Lyme Detection Tests:
Antigen detection: It spots the presence of an exclusive Bb protein in the patient’s fluid (urine). It is quite helpful to detect LD (Lime disease) in patients taking antibiotics or during severe symptom outbreak.PCR (Polimerase Chain Reaction): This test multiplies the figure of Bb DNA to a visible and measurable level.
Culturing: It is a bit difficult, as it not an easy task to nurture the bacterium.
Staining: It is a time consuming test and shows low yield. Here, the trouble is that in Limes disease, the number of Lime spirochete is low, and can result in the biopsy showing no bacteria.
One final and important thing that I would like to share with all you is that regular testing without symptoms imply lime disease infection, even in endemic regions, increases the chance of a positive test even without being infected.